相关实验视频
Updated: Jun 3, 2026
06:59
Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
比较 preQ1 рибо开关的溶液和晶体结构,揭示了诱导的变化在构造和动态
Qi Zhang1, Mijeong Kang, Robert D Peterson
1Department of Chemistry and Biochemistry , University of California, Los Angeles, California 90095-1569, USA.
Journal of the American Chemical Society
|March 18, 2011
概括
在 Bacillus subtilis preQ(1) 里波切换器上体表现出其结合体结合状态的内在灵活性. 在晶体结构中存在的离子对于其在溶液中的功能并不必不可少.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 带状开关是通过代谢物结合来调节基因表达的遗传元素.
- рибо开关的aptamer域结合特定的代谢物,诱导构造变化.
- 在 Bacillus subtilis preQ(1) 核突开关的溶液和晶体结构之间存在差异.
研究的目的:
- 为了在结构和动态上表征 Bacillus subtilis preQ(1) 用溶液NMR将其连接体结合状态中的 рибо开关阿巴.
- 为了研究离子在体结构和功能中的作用.
- 为了调和报告的溶液和晶体结构之间的差异.
主要方法:
- 解决方案核磁共振 (NMR) 光谱学. 解决方案核磁共振 (NMR) 光谱学.
- 亚胺-preQ(1) 复合体的结构和动态特征.
- 对离子的联结亲和度测量.离子.
主要成果:
- 在溶液中,阿普坦-前Q(1) 复合物本质上是灵活的,在不同区域具有明显的运动.
- 一个特定的离子与具有微分子亲和力的联体诱导口袋结合.
- 离子会诱导形状变化,但不需要在溶液中结合连接体或折叠.
结论:
- 细菌微的preQ(1) рибо交换机体在溶液中表现出显著的形状灵活性,即使与其连接物结合.
- 观察到的晶体和溶液结构之间的差异归因于离子相互作用.
- 离子对于溶液中的这种 рибо开关胺体的基本功能来说并不必不可少.
相关概念视频
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
01:23Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:44Termination of Translation
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...