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相关概念视频

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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相关实验视频

Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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使用条件RNA干扰研究小鼠基因功能的快速和可扩展的系统.

Prem K Premsrirut1, Lukas E Dow, Sang Yong Kim

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Cell
|April 5, 2011
PubMed
概括
此摘要是机器生成的。

我们开发了一种快速,可扩展的管道,用于生成RNAi转基因小鼠. 这种方法可以实现强大的基因沉默,并确定癌症的治疗点,加速研究.

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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
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相关实验视频

Last Updated: Jan 14, 2026

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07:52

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科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 转基因RNAi小鼠的可再生生成对基因功能研究至关重要,但仍然是一个重要的局限性.
  • RNA干扰 (RNAi) 为基因沉默提供了一种强大的方法.
  • 创建RNAi转基因小鼠的现有方法通常很慢,并且不容易扩展.

研究的目的:

  • 开发一种快速,可扩展和具有成本效益的生产shRNA转基因小鼠的管道.
  • 为了证明开发的基因沉默系统 in vivo 的有效性.
  • 使用RNAi技术识别癌症中的潜在治疗点.

主要方法:

  • 结合优化光合miR30基的shRNAs,具有高效的ES细胞向.
  • 产生了八个调节的shRNA转基因小鼠线,针对关键基因 (露西法酶,Oct4,p53,p16INK4a,p19ARF,APC).
  • 利用GFP跟踪可视化和量化各种组织中的基因淘汰.

主要成果:

  • 在体内广泛的组织中实现了强大的基因沉默和GFP追踪的敲击.
  • 证明了系统识别已知和新型基因功能的能力,以APC为例.
  • 验证了APC/Wnt和p19ARF作为T细胞急性淋巴细胞白血病/淋巴瘤和肺腺癌的潜在治疗点.

结论:

  • 开发的管道提供了一个具有成本效益和可扩展的平台,用于生产针对任何哺乳动物基因的RNAi转基因小鼠.
  • 这个系统显著推进了对基因功能的研究,并促进了治疗点的发现.
  • 这项技术有望加速各种领域的研究,包括癌症生物学和药物开发.