用酶控制和切换微粒纳米颗粒的形态
Ti-Hsuan Ku1, Miao-Ping Chien, Matthew P Thompson
1Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
Journal of the American Chemical Society
|April 6, 2011
概括
聚合物基因通过酶活性改变形状. 这项研究探讨了酸化和蛋白质分解如何控制微细胞形态,用于先进的纳米材料应用.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 纳米技术纳米技术
背景情况:
- 聚合物-块共聚合物为自组装提供可调节的特性.
- 酶响应材料对于向药物输送和诊断至关重要.
- 控制纳米粒子形态是它们功能的关键.
研究的目的:
- 为了设计具有酶可裂变和可修改的序列的聚合物-基.
- 为了研究由酶活性触发的这些细胞的可逆形态切换.
- 通过使用先进的成像,阐明统一的聚合物-纳米颗粒的内部结构.
主要方法:
- 聚合物-块共聚合物的合成与特定的基质.
- 自组装小组的制备和表征.
- 使用蛋白质激酶A,蛋白质酸酶-1和矩阵金属蛋白酶 (MMPs) 的酶治疗.
- 透电子显微镜 (cryo-TEM) 用于结构分析.
主要成果:
- 在酸化和脱酸化循环的反应中,菌体表现出可逆的形态变化.
- 由MMPs进行的序的蛋白质分解诱导了显著的,依赖于序列的小细胞形态的变化.
- 化TEM揭示了高度均的粒子,并提供了对它们内部组织的见解.
结论:
- 酶响应的聚合物-菌体可以经历受控的,可逆的形态转变.
- 这些材料显示出创造具有可调节性质的动态纳米结构的潜力.
- 该研究强调了冷-TEM在表征这些复杂的纳米材料方面的实用性.
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