对p34cdc2的环林激活
M J Solomon1, M Glotzer, T H Lee
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Cell
|November 30, 1990
概括
青蛋的环素积累触发了快速的p34cdc2激活,启动了线粒分裂. 这个由INH调节的过程涉及酸化和脱酸化动态,这对于细胞循环控制至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 青蛋中循环素的积累驱动了p34cdc2的激活,p34cdc2是启动线粒分裂的关键调节器.
- 这种激活过程表现出不寻常的动力学,包括在达到临界环林度后也存在延迟.
研究的目的:
- 在体外研究p34cdc2突然和延迟激活的机制.
- 描述INH (一种2A型蛋白酸酶) 在调节MPF (成熟促进因子) 激活中的作用.
主要方法:
- 利用细菌表达的环林蛋白和Xenopus蛋提取物进行体外实验.
- 分析了p34cdc2在氨酸和氨酸残留物上的酸化状态.
主要成果:
- 成功地重现了p34cdc2的突然激活,具有延迟期,独立于环素度.
- 证明INH可以调节值环林度和滞后期长度.
- 观察到环林结合导致p34cdc2酸化 (铁和三),导致不活化.
结论:
- 过渡到线粒分裂涉及p34cdc2酸化的协调变化,特别是降低的氨酸酸化和增加的脱酸化.
- 通过调节环林结合和p34cdc2酸化状态,INH充当MPF激活的关键调节者.
相关概念视频
01:45Positive Regulator Molecules
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02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
00:50Anaphase Promoting Complex
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02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...