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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

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相关实验视频

Updated: Jun 2, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

对于长非编码RNA的无意义功能.

Takashi Nagano1, Peter Fraser

  • 1Nuclear Dynamics and Function Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK.

Cell
|April 19, 2011
PubMed
概括
此摘要是机器生成的。

长非编码RNA,基因组的"暗物质",正在揭示复杂的功能. 最近的研究揭示了这些神秘分子在生物过程中的多样性作用.

相关实验视频

Last Updated: Jun 2, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 长非编码RNA (lncRNAs) 曾经被认为是转录噪声.
  • 它们在基因表达和细胞功能中的调节作用越来越被认可.
  • lncRNAs代表了真核生物基因组的一个重要,但尚未研究的组成部分.

研究的目的:

  • 阐明长非编码RNA的复杂和多样化的功能.
  • 突出最近在理解 lncRNA 机制方面的进展.
  • 为了探索基因组的"暗物质".

主要方法:

  • 对 lncRNA 序列和表达数据的生物信息分析.
  • 功能性基因组学研究,包括淘汰和过度表达实验.
  • 高通量测序技术用于识别新型 lncRNA及其目标.

主要成果:

  • 识别具有特定细胞作用的新 lncRNAs.
  • 通过 lncRNAs 调节基因表达的各种机制的表征.
  • 有证据表明lncRNAs参与各种生物途径,包括发育和疾病.

结论:

  • 长非编码RNA具有超出简单基因调节的多方面的功能.
  • 这些分子是细胞过程和疾病发病过程中的关键参与者.
  • 对lncRNAs的进一步研究有望对基因组功能产生重大见解.