氧气进化的光系统II的晶体结构,分辨率为1.9 Å
Yasufumi Umena1, Keisuke Kawakami, Jian-Ren Shen
1Department of Chemistry, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka 558-8585, Japan.
Nature
|April 19, 2011
概括
研究人员确定了光系统II的1.9 Å晶体结构,揭示了对水氧化至关重要的-集群的详细安排. 这种高分辨率结构照亮了催化部位和潜在的水基板通道.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 光合作用研究研究 光合作用研究
背景情况:
- 光系统II (PSII) 进行光合作用水氧化.
- 之前的PSII结构 (2.93.8 Å) 缺乏催化水分裂中心的细节.
研究的目的:
- 为了确定光系统II的高分辨率晶体结构.
- 为了阐明水分裂催化中心的详细结构.
主要方法:
- 在X射线晶体学.
- 照片系统II的高分辨率 (1.9 Å) 结构确定.
主要成果:
- 详细的原子结构的Mn{4) CaO{5) 集群及其连接体.
- 在催化部位识别了五个氧桥和四个结合的水分子.
- 发现了1300多个水分子,形成潜在的质子/水/氧气通道.
结论:
- 1.9 Å结构为光系统II催化中心提供了前所未有的细节.
- 已识别的水分子可以作为二氧化物形成的基质.
- 观察到的水网络表明分子运输的道,有助于功能分析.
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