一个单基因嗅觉受体表达的表观遗传特征
Angeliki Magklara1, Angela Yen, Bradley M Colquitt
1Department of Anatomy, University of California, San Francisco, San Francisco, CA 94158, USA.
Cell
|May 3, 2011
概括
构成性异质色素在小鼠嗅觉表皮中动态标记嗅觉受体 (OR) 基因. 这种动态色素修饰,涉及H3K9me3和H4K20me3,在OR基因表达之前,挑战了以前的OR选择模型.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 构成性的异色染色体通常被认为是静态的,沉默重复的DNA元素.
- 嗅觉受体 (OR) 基因表达受到复杂机制的调节,确保每个神经元的独特,单基因表达.
研究的目的:
- 为了研究OR基因位置的构成性异色素标记的动态性质.
- 阐明这些标记在OR选择和表达规范过程中的作用.
主要方法:
- 分析小鼠嗅觉上皮的H3K9me3和H4K20me3标记.
- 研究这些表观遗传标记的细胞类型和发育依赖性.
- 与OR基因表达模式相关的染色质状态.
主要成果:
- 嗅觉受体 (OR) 基因由动态构成性异色素特征标记 (H3K9me3,H4K20me3).
- 这些标记以细胞类型和发育依赖的方式存储.
- 这些标记的沉积和可能的反转在OR表达之前,暗示了先发制人的沉默.
结论:
- 染色体介导的沉默,而不是反信号,可能会先于OR基因表达.
- 动态色素在建立单基OR表达的基础上起着至关重要的作用.
- 这挑战了对构成性异性染色素纯粹静态的传统观点.
更多相关视频
10:16Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
Published on: July 13, 2015
06:32Hybridization Chain Reaction RNA Whole-Mount Fluorescence In situ Hybridization of Chemosensory Genes in Mosquito Olfactory Appendages
Published on: November 17, 2023
相关概念视频
01:03Olfactory Receptors: Location and Structure
The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
01:25Olfaction
The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...
The olfactory receptors are embedded in the cilia of the...
02:30Genomic Imprinting and Inheritance
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...