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Updated: Jul 12, 2026

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

在体外分辨率的协同连接的AAV染色体末端

R O Snyder1, R J Samulski, N Muzyczka

  • 1Department of Microbiology, SUNY Stony Brook Medical School 11794.

Cell
|January 12, 1990
PubMed
概括

研究人员开发了一种用于腺相关病毒 (AAV) DNA复制的新检测方法. 这种试验证明了共价连接的AAVDNA末端的体外分离,为关键的复制步骤提供了生化证据.

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Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...