Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Organization01:24

Protein Organization

10.0K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
10.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Lysozyme revisited: evaluating models of a reference protein in structural biology.

Current research in structural biology·2026
Same author

Impact of Single Halogen Atom Substitutions on Antiviral Profile of Inhibitors Targeting SARS-CoV‑2 Main Protease.

ACS omega·2026
Same author

How to mitigate the caveat emptor burden of human and machine users of the Protein Data Bank.

Acta crystallographica. Section D, Structural biology·2026
Same author

A Critical Look at the Crystal Structures of cAMP-Dependent Protein Kinases.

Kinases and phosphatases·2025
Same author

Activity and structure of human (d)CTP deaminase CDADC1.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

A Bifunctional Phosphoglucomutase/Phosphomannomutase from <i>Thermococcus kodakarensis</i>: Biophysical Analysis and Cryo-EM Structure.

Biomolecules·2025

相关实验视频

Updated: Mar 29, 2026

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

965

通过密度和能量导向蛋白质结构优化改进分子替换.

Frank DiMaio1, Thomas C Terwilliger, Randy J Read

  • 1University of Washington, Department of Biochemistry and HHMI, Seattle, Washington 98195, USA.

Nature
|May 3, 2011
PubMed
概括

通过将蛋白质结构建模与晶体学方法相结合,可以解决困难的蛋白质晶体结构. 这种新方法克服了传统分子替代的局限性,提高了蛋白质结构确定成功率.

更多相关视频

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.7K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

70.1K

相关实验视频

Last Updated: Mar 29, 2026

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function
05:57

Author Spotlight: In Silico Creation and Impact of Carbonylated Amino Acids on Protein Structure and Function

Published on: April 26, 2024

965
Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.7K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

70.1K

科学领域:

  • 结构生物学 结构生物学
  • 计算生物学 计算生物学
  • 晶体学 晶体学是指结晶学.

背景情况:

  • 分子替代 (MR) 和链跟踪是蛋白质结构确定的标准.
  • 当前的MR方法通常在与已知结构相同的序列相同性<30%的模型中失败.

研究的目的:

  • 开发一种新的方法来克服蛋白质晶体结构确定方面的局限性.
  • 通过使用X射线衍射数据来提高解决蛋白质结构的成功率.

主要方法:

  • 集成的罗塞塔结构建模与自动构建链条跟踪算法.
  • 应用了组合方法来挑战X射线衍射数据集.

主要成果:

  • 成功确定了13个困难数据集中的8个的高分辨率结构.
  • 该方法在专家结晶学家和其他方法失败的地方取得了成功.
  • 这种方法对超过一半以前无法解决的案件显示出希望.

结论:

  • 结合蛋白质结构建模和晶体算法,显著提高了蛋白质结构的确定.
  • 这种综合方法扩大了可以通过X射线晶体学解决的情况范围,而无需实验分相.
  • 该技术为推进结构生物学研究提供了一个强大的工具.