相关实验视频

Updated: Jul 30, 2026

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Published on: March 2, 2018

关于NCAM主要异型对神经元外生长的值效应

P Doherty¹, M Fruns, P Seaton

¹Department of Neurochemistry, Institute of Neurology, London, UK.

|February 1, 1990

概括

神经细胞粘附分子 (NCAM) 异型促进神经细胞外生长. 需要NCAM表达的值水平,此后增加表达显著提高神经元的生长和指导.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
发展生物学发展生物学

背景情况:

神经细胞外生长对于神经发育和目标内化至关重要.
神经细胞粘附分子 (NCAM) 在指导神经炎中的确切作用尚不清楚.
之前的研究给出了关于NCAM在神经元扩展中的作用的相互矛盾的结果.

研究的目的:

研究不同神经细胞粘附分子 (NCAM) 异型在神经细胞外生长中的作用.
为了确定NCAM表达水平和神经元扩展之间的剂量反应关系.
分析NCAM表达对神经元生长的形态影响.

主要方法:

在表达各种NCAM异型的3T3细胞单层上培养大鼠小脑神经元.
使用体外测试来评估神经元外生长.
对不同NCAM表达水平的反应中神经元生长模式的形态分析.

主要成果:

跨膜和与葡萄糖酸氨基醇相关的NCAM异型都支持神经元扩展.
需要NCAM表达的临界值来增强神经细胞外生长.
神经细胞外生长大幅增加,NCAM表达的微小升高超过了值.

结论:

NCAM异型是神经元扩张的有力基质.
神经细胞外生长取决于达到NCAM表达的特定值.
NCAM在调节神经元路径和连接方面发挥着重要作用.

更多相关视频

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

Published on: October 19, 2019

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Trigeminal Ganglia

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Trigeminal Ganglia

Published on: August 1, 2025

相关概念视频

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...