在光合作用藻中尿素循环的演变和代谢意义
Andrew E Allen1, Christopher L Dupont, Miroslav Oborník
1J. Craig Venter Institute, San Diego, California 92121, USA. aallen@jcvi.org
Nature
|May 13, 2011
概括
藻利用一种由外共生生物衍生的甲素-尿素循环,以快速从限度中恢复. 这条对海洋生产力至关重要的途径,将细菌和外生生物基因连接起来,以实现重要的代谢功能.
科学领域:
- 海洋生物学 海洋生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 藻是生产性海洋食物网中的关键植物浮游生物.
- 它们的基因组包含来自细菌,塑体和二级内共生宿主 (外共生动物) 的基因.
- 这些基因组对藻的代谢贡献在很大程度上是未知的.
研究的目的:
- 调查外共生生物衍生的花素-尿素循环在藻代谢中的作用.
- 确定其对利用和回收的贡献.
- 探索外共生体,细菌和宿主衍生的途径之间的代谢联系.
主要方法:
- RNA干扰 (RNAi) 来击败线粒体碳酸合成酶.
- 代谢分析以评估代谢物水平.
- 进行比较基因组学以了解基因起源.
主要成果:
- 基酸盐合成酶的抑制损害了有限的藻对添加的反应.
- 甲素-尿素循环中间体被耗尽,将其与三碳酸和谷氨酸合成酶/谷氨酸合成酶循环联系起来.
- 代谢物,包括プロ林和聚胺前体,通过结合的细菌和外共生生物通路产生.
结论:
- 藻的甲素-尿素循环有助于从限度中快速恢复.
- 它作为无机碳和分布的枢纽,对藻生长至关重要.
- 这一循环对于藻对海洋生产力的贡献和适应营养物质可用性的波动至关重要.
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