在海洋细菌中广泛存在的二甲基硫 propionate 的新吸收途径
Chris R Reisch1, Melissa J Stoudemayer, Vanessa A Varaljay
1Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
Nature
|May 13, 2011
概括
海洋细菌利用一种新的酶途径来处理二甲基硫 propionate (DMSP),将二甲基 propionate (MMPA) 同化并产生二甲 (MeSH). 这种途径在海洋细菌浮游生物中很普遍.
科学领域:
- 海洋微生物生态学
- 生物地质化学生物地质化学
- 酶学 是一种酶学.
背景情况:
- 甲基硫 propionate (DMSP) 是海洋表面水中的一个关键的碳和硫化合物,由植物浮游生物产生.
- 海洋细菌通过脱甲基化/脱甲基化处理DMSP,释放甲乙醇 (MeSH) 并吸收减少的硫.
- 超过二十年来,甲基甲 propionate (MMPA) 吸收的特定酶和生化途径仍然是未知的.
研究的目的:
- 为了确定生物化学途径和酶,负责从DMSP分解的甲基甲 propionate (MMPA) 的同化.
- 阐明海洋环境中减少硫的微生物同化机制.
主要方法:
- 使用遗传和酶分析进行生化途径重建.
- 新型协酶A (CoA) 衍生物的鉴定:3-甲基烯烯-CoA (MMPA-CoA) 和甲基烯-CoA (MTA-CoA).
- 对细菌菌株 (例如,Ruegeria pomeroyi DSS-3,Pelagibacter ubique) 和海洋细菌浮游生物的基因组和基因组分析.
主要成果:
- 与脂肪酸β-氧化相关的三种新型酶被确定为MMPA同化途径.
- 该途径涉及MMPA-CoA和MTA-CoA作为关键中间体的形成.
- 这条途径对于Ruegeria pomeroyi中的MMPA同化和MeSH产生至关重要,并且在海洋细菌浮游生物中广泛存在.
结论:
- 一个以前未知的微生物途径涉及脂肪酸β-氧化酶,同化MMPA,一种DMSP衍生的化合物.
- 这一途径对于海洋异质动物获取硫和碳至关重要.
- 已确定的路径代表了在海洋表面水中减少硫循环的重要路径.
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