NLRP6炎症酶调节结肠微生物生态和结肠炎风险
Eran Elinav1, Till Strowig, Andrew L Kau
1Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Cell
|May 14, 2011
概括
在小鼠中的NLRP6炎症酶缺乏会改变肠道微生物群,增加对结肠炎的易感性. 这种微生物群.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 炎症细胞是损伤信号的关键免疫传感器.
- 在肠道平衡中NLRP6炎症酶的作用尚未完全理解.
研究的目的:
- 研究结肠中的NLRP6炎症酶功能的研究.
- 确定NLRP6缺乏对肠道微生物群和结肠炎易感性的影响.
主要方法:
- 缺乏NLRP6的小鼠模型
- 便微生物群分析 (16S rRNA测序)
- 德克斯硫酸 (DSS) 诱导的大肠炎模型
- 交叉培养和共同住房实验.
- 细胞因子分析 (CCL5)
- 使用抗生素治疗治疗.
- 电子显微镜的电子显微镜
主要成果:
- 缺乏NLRP6会降低IL-18水平,并改变了微生物群组成 (扩大Bacteroidetes/Prevotellaceae,TM7).
- 缺乏NLRP6的小鼠表现出自发性肠道增生和恶化的DSS大肠炎.
- 来自NLRP6缺乏的小鼠的可转移性胆固醇菌群通过CCL5诱导在野生类型接受者中加剧了DSS胆固醇炎.
- 鉴定出Prevotellaceae是微生物群相关表型的一个关键贡献者.
结论:
- NLRP6炎症酶对维持肠道平衡和调节微生物群组成至关重要.
- NLRP6炎症酶通路和相关微生物群的失调可能会导致炎症性肠病 (IBD).
- 针对炎症酶-微生物群相互作用可以为IBD提供治疗策略.
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