细胞史对对神经调节剂螺旋环螺旋家族的反应的影响
B W Schäfer1, B T Blakely, G J Darlington
1Department of Pharmacology, Stanford University School of Medicine, California 94305-5332.
Nature
|March 29, 1990
概括
肌肉特异性基因调节剂MyoD和myogenin可以诱导纤维细胞中的肌肉基因表达,但不能诱导肝细胞. 纤维细胞中的其他因素是细胞融合中肌肉基因完全激活所需的.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 发育生物学是发展生物学.
背景情况:
- 不同化的细胞在体内保持稳定的状态.
- 细胞融合可以导致基因表达和细胞可塑性的改变.
- 从肌肉细胞和其他细胞类型中形成的异构体显示出改变的基因表达模式.
研究的目的:
- 调查MyoD或myogenin是否可以诱导非肌肉细胞中的肌肉特异性基因表达.
- 确定这些调节剂在异构体中观察到的表型转换中的作用.
- 确定细胞融合过程中肌肉基因激活所需的因素.
主要方法:
- 通过微注射,转感染和逆转病毒感染稳定和短暂地将MyoD/myogenin引入纤维细胞和肝细胞.
- MyoD表达性肝细胞与纤维细胞的融合.
- 分析生成的异构体中的肌肉特异性和肝脏特异性基因表达.
主要成果:
- MyoD/myogenin表达诱导了纤维细胞中的肌肉特异基因,但不是肝细胞.
- MyoD表达性肝细胞与纤维细胞的融合激活了异质细胞内两种细胞类型的肌肉特异基因.
- 当直接引入MyoD/myogenin时,肝细胞没有激活肌肉特异性基因.
结论:
- 单独MyoD和myogenin不足以诱导肝细胞中的肌肉特异性基因表达.
- 除了MyoD/myogenin外,还需要纤维细胞特异性因素来进行强大的肌肉基因激活.
- 细胞分化状态受到细胞史和蛋白质相互作用的影响.
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