诱导多能干细胞的免疫性
Tongbiao Zhao1, Zhen-Ning Zhang, Zhili Rong
1Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0322, USA.
Nature
|May 17, 2011
概括
诱导多能干细胞 (iPSCs) 可以触发受体的免疫反应,这与自身治疗的预期相反. 这项研究表明,与胚胎干细胞 (ESC) 不同的是,iPSC衍生细胞可以具有免疫性,因此需要进行临床前评估.
科学领域:
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
背景情况:
- 诱导多能干细胞 (iPSCs) 是再生医学的一个有希望的来源,因为它们的自身潜力.
- 一般认为iPSC衍生细胞具有免疫耐受性,但其免疫性需要严格检查.
研究的目的:
- 调查胚胎干细胞 (ESC) 和诱导多能干细胞 (iPSC) 在同源性小鼠模型中的免疫性.
- 确定iPSC衍生细胞是否会在宿主中引起免疫反应,这可能会影响它们的治疗应用.
主要方法:
- 使用全基因和同基因ESCs和iPSCs (通过逆转录病毒和插曲方法获得) 进行了瘤形成测试.
- 通过观察瘤生长,免疫细胞透 (T细胞),组织损伤和回归来评估免疫性.
- 全球基因表达分析对瘤进行,以确定差异表达的基因.
主要成果:
- 全基因ESC被拒绝,而同基因ESC在没有免疫排斥的情况下形成了瘤.
- 来自综合性iPSCs (逆转录病毒和病例性) 的瘤显示出显著的免疫排斥和T细胞透.
- 基因表达分析揭示了iPSC衍生的瘤中特定基因的过度表达,这有助于免疫性.
结论:
- 与ESC不同,iPSC衍生细胞可以诱导T细胞依赖的免疫反应.
- 在差异化的iPSC中异常的基因表达可以导致免疫性.
- 患者特异性iPSC衍生细胞的治疗应用需要在临床使用之前对其免疫性进行彻底评估.
相关概念视频
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