完成原始模型细胞的芽和不对称的分裂,以产生具有不同内部和膜组成的子囊泡
Meghan Andes-Koback1, Christine D Keating
1Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Journal of the American Chemical Society
|May 20, 2011
概括
简单的模型细胞,模仿生物系统,实现了不对称的分裂. 这些无生命的巨型脂质囊泡 (GVs) 产生了化学上不同的子囊泡,为细胞分裂起源提供了洞察力.
科学领域:
- 生物物理学的生物物理.
- 化学生物学 化学生物学
- 生命的起源研究 生命的起源研究
背景情况:
- 不对称的细胞分裂是生物学中差异化和发育的基础.
- 单细胞生物可以作为研究细胞分裂不对称性的模型,但即使是这些生物也很复杂.
- 要了解细胞分裂的基本原理,需要简单,无生命的模型系统.
研究的目的:
- 创造和研究简单的,无生命的模型细胞,能够进行不对称的分裂.
- 研究由这些模型细胞产生的子囊泡的化学和形态特异性.
- 探索脂质域和内部相分离在驱动不对称细胞分裂中的作用.
主要方法:
- 使用巨型脂质囊泡 (GVs) 封装聚乙烯糖醇 (PEG) /德克斯水性双相系统 (ATPS) 作为细胞质模仿.
- 嵌入的三元脂质组合物以创建共存的液体无序 (L(d)) 和液体有序 (L(o)) 膜域.
- 诱导芽和裂变通过通过糖添加的奥斯莫斯压力变化.
主要成果:
- 实现了模型细胞的完全芽和不对称裂变,产生具有独特内部和膜组成的子囊泡.
- 证明分离发生在水性-水性相界,将富含PEG和富含德克斯的相分离为不同的女儿相.
- 表明L ((o) 域中的PEG化脂质优先覆盖PEG丰富的芽,多余的膜域被特定的女儿继承.
结论:
- 简单的自组装系统可以表现出复杂的行为,如不对称的裂变,产生不同组成的子囊泡.
- 这些含有ATPS的GV为研究非对称细胞分裂和遗传中的空间和组织线索提供了可测试的平台.
- 这些发现表明,分子组装的基本原理可以成为细胞分裂等生物过程的基础.
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