伊诺西1,2-环酸盐2-酸酶与利波丁III的身份
T S Ross1, J F Tait, P W Majerus
1Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110.
概括
人类胎盘内素1,2-环酸2-酸,细胞信号中的一种酶,与利波丁III共享序列. 这表明,脂质皮质素III在酸氨基醇途径中可能发挥作用.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 氨酸的信号通路对于细胞功能至关重要.
- 利波丁III,也称为胎盘抗凝蛋白III (PAP III),是一种和脂结合蛋白,具有未定义的生理作用.
- 伊诺西1,2-环酸2-酸酶是酸丁氨基信号通路中的一种酶.
研究的目的:
- 调查人类胎盘内醇1,2-环酸二酸和利波丁III之间的关系.
- 探索皮蛋白III在酸氨基醇信号通路中的潜在功能.
主要方法:
- 原化物消化人类胎盘内醇1,2-环酸盐2-酸.
- 酶碎片的氨基酸序列分析.
- 使用对胎盘抗凝蛋白III (PAP III) 的抗体进行免疫血栓.
- 在酸性脂的存在下评估酶活性.
主要成果:
- 三个伊诺西1,2-环酸2-酸酶的碎片显示出与利波丁III相同的氨基酸序列.
- 对PAP III的抗体与PAP III和伊诺西1,2-环酸盐2-酸酶进行了交叉反应.
- 伊诺西1,2-环酸2-酸酶活性是由已知的酸性脂激活,以结合lipocortins.
结论:
- 人类胎盘内醇1,2-循环酸盐2-酸酶与脂质皮质素III/PAPIII相同.
- 利波丁III/PAP III可能在酸氨基醇信号通路中发挥作用.
- 这一发现为利波丁III提供了潜在的生理功能.
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