通过BPTF通过多价值相互作用识别 mononucleosomal histone 修饰模式
Alexander J Ruthenburg1, Haitao Li, Thomas A Milne
1Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.
Cell
|May 21, 2011
概括
组合的基因组修饰是染色质功能的关键. 这项研究确定了一种特定的组织蛋白标记 (H3K4me3和H4K16ac) 模式,该模式指导蛋白质与核细胞结合.
科学领域:
- 染色体生物学 染色体生物学
- 分子表观遗传学 分子表观遗传学
- 蛋白质-DNA相互作用
背景情况:
- 基因组蛋白修饰调节基因表达和染色质结构.
- 该BPTF蛋白的PHD指可以识别甲基化素H3 (H3K4me2/3).
- 组合的基因素标记如何影响核细胞层面的蛋白质结合在很大程度上是未知的.
研究的目的:
- 为了研究额外的质子修饰如何影响BPTF结合.
- 为了识别由BPTF识别的特定的组合基因素标记.
- 了解核体标记模式在染色体协会中的作用.
主要方法:
- 对替代物的系统选.
- 蛋白质-配体相互作用的生物物理特征.
- 单核细胞结合试验. 单核细胞结合试验.
- 全基因组的同地化分析.
主要成果:
- 在BPTF中确定了一种与PHD相邻的基因,可以结合三种乙氨酸.
- 只有在与H3K4me3结合时,BPTF基因在单核细胞水平上才对H4K16ac表现出选择性.
- 这种H3K4me3和H4K16ac模式被证实是人类细胞单核细胞内的独特的转基因基因修饰.
- 这种特定的基因素标记模块与基因组中的BPTF结合点共定位.
结论:
- 核标记的核体模式决定了关键的染色体关联.
- 在单个核酶体上H3K4me3和H4K16ac的组合是BPTF的特定识别信号.
- 这一发现凸显了考虑多个组织蛋白标记的相互作用对于理解染色体调节的重要性.
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