Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

8% capsaicin patches for HIV-induced polyneuropathy: a systematic review and meta-analysis.

Pain management·2026
Same author

Refractory Chronic Neuropathic Pain Successfully Treated With Scrambler Therapy.

The primary care companion for CNS disorders·2026
Same author

Intrathecal baclofen pump failure due to pain-induced autonomic dysreflexia.

BMJ case reports·2026
Same author

Evaluation of the Gigstride wearable device for pain reduction and functional outcomes in patients with lower extremity mobility deficits: a single-arm pilot feasibility study.

Pain management·2026
Same author

Inferior Outcomes of p230-CML with Imatinib: a Report of 2 Cases of p210/p230 Co-Expressed CML Showing Rapid Progression To Leukaemia Cutis along with the Systematic Review.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2026
Same author

Polyamine homeostasis in Caenorhabditis elegans relies primarily on transport.

Cell reports·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
查看所有相关文章

相关实验视频

Updated: May 5, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

10.9K

使用单分子拉下拉下作用探测细胞蛋白质复合体.

Ankur Jain1, Ruijie Liu, Biswarathan Ramani

  • 1Center for Biophysics and Computational Biology and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Nature
|May 27, 2011
PubMed
概括
此摘要是机器生成的。

这项研究引入了单分子拉下 (SiMPull) 试验,以可视化细胞内的单个蛋白质复合体. 这种方法揭示了蛋白质组合在各种生物途径中的组成和功能.

更多相关视频

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.1K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

14.2K

相关实验视频

Last Updated: May 5, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

10.9K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.1K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

14.2K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 蛋白质通过宏分子复合体来执行细胞功能.
  • 蛋白质经常参与多个复合体,从而产生不同的功能.
  • 现有的方法很难在体内捕捉蛋白相互作用的动态变换.

研究的目的:

  • 开发一种用于可视化和分析单个细胞蛋白质复合物的新型试验.
  • 克服组合方法在研究蛋白质相互作用排列的局限性.
  • 为了能够直接可视化蛋白质复合物的组成和石化测量.

主要方法:

  • 单分子拉下 (SiMPull) 试验的开发.
  • 传统的拉下技术与单分子光显微镜的整合.
  • 适用于来自动物组织的各种信号蛋白和内源复合体.

主要成果:

  • SiMPull 可以直接可视化单个蛋白质复合体.
  • 该试验准确地确定了复合体内的蛋白质的数量和类型,证明了蛋白质激酶A.
  • 在细胞区和内源性提取物中证明了广泛的适用性.
  • 拉下来的蛋白质在随后的单分子生物化学研究中仍然具有功能.

结论:

  • SiMPull是一个灵敏,快速和强大的平台,用于分析蛋白质组合.
  • 为蛋白质复合体的组成和动态提供了前所未有的洞察力.
  • 促进对生物通路中蛋白质复合体作用的更深入的理解.