Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evaluation of the properties of daughter bubbles generated by inertial cavitation of preformed microbubbles.

Ultrasonics sonochemistry·2020
Same author

Graph Adaptation Network with Domain-Specific Word Alignment for Cross-Domain Relation Extraction.

Sensors (Basel, Switzerland)·2020
Same author

Membrane Damage during Ferroptosis Is Caused by Oxidation of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1.

Molecular cell·2020
Same author

Printing special surface components for THz 2D and 3D imaging.

Scientific reports·2020
Same author

The role of post-loss anxiety in the development of depressive symptoms and complicated grief symptoms: a longitudinal SEM study.

Journal of affective disorders·2020
Same author

Enhancement of PAHs biodegradation in biosurfactant/phenol system by increasing the bioavailability of PAHs.

Chemosphere·2020

相关实验视频

Updated: Jun 1, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

颜色测量细菌感应使用一个超分子酶-纳米粒子生物传感器.

Oscar R Miranda1, Xiaoning Li, Limary Garcia-Gonzalez

  • 1Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.

Journal of the American Chemical Society
|June 2, 2011
PubMed
概括

一个新的酶-纳米粒子系统使微生物污染的敏感色度检测成为可能. 该方法量化了溶液中的细菌和测试带上的细菌,这对于环境和临床病原体检测至关重要.

更多相关视频

Colorimetric Detection of Bacteria Using Litmus Test
10:05

Colorimetric Detection of Bacteria Using Litmus Test

Published on: September 17, 2016

相关实验视频

Last Updated: Jun 1, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
13:20

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Colorimetric Detection of Bacteria Using Litmus Test
10:05

Colorimetric Detection of Bacteria Using Litmus Test

Published on: September 17, 2016

科学领域:

  • 纳米技术纳米技术
  • 生物化学 生物化学
  • 微生物学 微生物学

背景情况:

  • 快速和敏感的病原体检测对于环境和临床应用至关重要.
  • 目前的方法可能缺乏某些设置所需的灵敏度或速度.

研究的目的:

  • 为微生物污染检测开发色度酶-纳米粒子结合系统.
  • 使用这种新的方法,实现细菌的敏感和快速量化.

主要方法:

  • 采用阴阳金纳米颗粒 (NP) 静电结合β-galactosidase (β-Gal) 抑制酶活性.
  • 分析细菌与NP结合释放β-Gal,恢复色度读数的酶活性.
  • 开发了一个实地友好的测试带格式,用于实际应用.

主要成果:

  • 量化细菌的度低至1 × 10 ((2) 细菌/毫升在溶液中.
  • 使用试卷格式实现了1 × 10(4) 细菌/毫升的检测极限.
  • 证明了一种与细菌存在成比例的酶增强色度信号.

结论:

  • 酶-纳米粒子系统为检测微生物污染提供了灵敏和快速的方法.
  • 这种方法可以适应基于解决方案的测试和现场部署的测试条.
  • 该技术对环境监测和临床诊断具有前景.