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Updated: Jun 1, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

单个细胞的时空多色标记使用功能化量子点和混合交付技术

James B Delehanty1, Christopher E Bradburne, Kimihiro Susumu

  • 1Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC 20375, USA. james.delehanty@nrl.navy.mil

Journal of the American Chemical Society
|June 2, 2011
PubMed
概括

研究人员开发了一种基于纳米粒子的新方法,用于对活细胞进行多色光标签. 这种技术允许同时对多个亚细胞结构进行成像,而无需细胞固定或使用抗体.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...