复合原始XMRV复原病毒的复合原始
Tobias Paprotka1, Krista A Delviks-Frankenberry, Oya Cingöz
1Viral Mutation Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
概括
异源性小鼠白血病病毒相关病毒 (XMRV) 可能是由于在瘤传递过程中在小鼠中两种前病毒的重组而产生的. 这表明XMRVV.
科学领域:
- 病毒学 病毒学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 在人体样本中检测异型鼠类白血病病毒相关病毒 (XMRV) 存在争议,原因是缺乏复制.
- 之前的研究报告XMRV在前列腺癌和慢性疲劳综合征患者.
- XMRV的起源和生成机制仍然不清楚.
研究的目的:
- 为了调查XMRV的起源和生成.
- 了解XMRV如何在人类前列腺癌细胞系和异种移植中出现.
- 解释关于人类样本中XMRV存在的不一致发现.
主要方法:
- 研究了人类前列腺癌细胞系 (CWR22Rv1,CWR-R1) 和它们的原始异种移植 (CWR22) 通过在小鼠.
- 分析了异种移植和细胞系的早期和晚期通道中的病毒存在.
- 测序了宿主小鼠的前病毒 (PreXMRV-1,PreXMRV-2),并将它们与XMRV进行了比较.
主要成果:
- 在CWR22Rv1,CWR-R1细胞系和晚期通道异种移植中检测到XMRV,但在早期通道中没有检测到.
- 确定了两个小鼠前病毒,PreXMRV-1和PreXMRV-2,与XMRV共享99.92%的同一性.
- 原始CWR22瘤中没有XMRV,这表明它是在通过过程中产生的.
结论:
- 瘤传递过程中,XMRV起源于两只小鼠前病毒之间的重组事件.
- 这种完全相同的重组的独立生成是极不可能的.
- 人体样本被这种小鼠产生的XMRV污染可能解释了它与人类疾病的关联.
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