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在粗的内分泌网膜中识别了核糖体受体

A J Savitz¹, D I Meyer

¹Department of Biological Chemistry, UCLA School of Medicine 90024.

|August 9, 1990

概括

研究人员确定了一种关键的蛋白质,该蛋白质介导着核糖体附着在内质网膜上. 这种蛋白质的蛋白质.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
蛋白质的运输蛋白质的运输

背景情况:

核糖体附着于内质网膜 (ER) 膜对于哺乳动物细胞中的蛋白质分泌至关重要.
这个过程是由特定的不可分割的膜蛋白调解的.

研究的目的:

为了识别和描述负责核糖体膜附着的蛋白质.
为了研究这种受体在核糖体结合中的细胞质域的作用.

主要方法:

细胞质域从ER膜中的蛋白质分解分离.
隔离,净化和表征180K整体膜蛋白质.
净化受体的功能复制成脂质囊泡.

主要成果:

一种18万个相对分子质量 (Mr 180K) 的整体膜蛋白被确定为核糖体受体.
这种蛋白质的大型细胞质域 (至少160K) 可以竞争性地抑制核糖体结合.
隔离的受体成功地被重组成脂质囊泡,证明了它的功能.

结论:

180K蛋白质是主要的受体,介导着核糖体附着于ER膜.
细胞质域在调节核糖体结合中起着至关重要的作用.
功能性复制验证了蛋白质在蛋白质运输中的作用.

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