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一个结合触发的DNA酶级联用于放大光检测具有零背景信号的生物小分子

Li-Min Lu1, Xiao-Bing Zhang, Rong-Mei Kong

  • 1State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha 410082, P. R., China.

Journal of the American Chemical Society
|June 14, 2011
PubMed
概括

一种新的结合触发的DNA酶级联为检测ATP和NAD等生物小分子提供了超高的灵敏度和选择性. 这种方法克服了以前生物传感器的局限性,使其能够与同类产品进行精确的区分.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...