互锁的前循环控制了Drosophila眼中的细胞类型特定的Rhodopsin表达
Robert J Johnston1, Yoshiaki Otake, Pranidhi Sood
1Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA.
Cell
|June 14, 2011
概括
复杂的基因网络控制细胞的发育. 在Drosophila眼中,缺陷的proventriculus (dve) 基因充当关键调节器,使用对立的前循环来精确确定光受体细胞类型.
科学领域:
- 发育生物学是发展生物学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 细胞类型的确定对于发育至关重要.
- 罗多普辛表达模式定义了Drosophila眼中的光受体亚型.
- 规范这一特点的监管网络尚未完全理解.
研究的目的:
- 调查转录因子基因缺陷的proventriculus (dve) 在调节Rhodopsin表达中的作用.
- 为了阐明控制Drosophila眼中的细胞类型决定的网络架构.
主要方法:
- 对涉及转录因子的基因调节网络的分析.
- 调查前循环 (FFL) 机制.
- 研究Drosophila光受体中的基因表达模式.
主要成果:
- Dve 在调节Rhodopsin表达的网络中充当关键节点.
- 一个不连贯的FFL涉及Ortodenticle和Dve抑制了Rhodopsin的表达.
- 在R7和R8光受体中的一致的FFL缓解了Dve介导的抑制,并激活了Rhodopsin表达.
- 两个级别为细胞类型和区域特定的结果进行了微调.
结论:
- 调节网络利用抑制来限制和组合激活来诱导细胞类型特定的Rhodopsin表达.
- 互锁的FFL代表了控制终端细胞酸盐规范的潜在通用机制.
相关概念视频
01:11Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
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