一种真菌高降解聚基酸合成酶的非遗传重编程
Ahmed A Yakasai1, Jack Davison, Zahida Wasil
1School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.
Journal of the American Chemical Society
|June 17, 2011
概括
研究真菌代谢物林的生物合成,发现了除了典型的林之外的多种产品. 表观遗传修饰剂和基因沉默揭示了新的化合物,突出的是多基合成酶.
科学领域:
- 生物化学 生物化学
- 菌群学 菌群学 菌群学是指菌群学
- 合成生物学 合成生物学
背景情况:
- 菌代谢物特内林是由贝奥维里亚巴西安纳 (Beauveria bassiana) CBS110.25.25产生的,它可以在菌中产生.
- 了解素生物合成对于探索真菌的二次代谢物生产至关重要.
- 表观遗传调节在微生物的二次新陈代谢中起作用.
研究的目的:
- 在Beauveria bassiana CBS110.25.25中研究林的生物合成.
- 探索表观遗传修饰剂 (5-azacytidine和suberoyl bis-hydroxamic acid) 对特内林生产的影响.
- 确定特内林生物合成基因集群中的单个基因沉默对产品形成的影响.
主要方法:
- 在不同的条件下种植Beauveria bassiana CBS110.25.
- 用表观遗传修饰剂5-azacytidine和suberoyl bis-hydroxamic acid进行治疗.
- 在林生物合成基因集群中的单个基因的沉默.
- 合成化合物的化学分析和结构阐明.
主要成果:
- 合成了许多新的化合物,证明了除了烯之外的多种可能产品.
- 这项研究表明,素生物合成不是一条固定的途径,而是可以产生多种结果.
- 对新产品的结构分析提供了有关林聚基酸合成酶的选择性的见解.
结论:
- 特内林的生物合成比以前所认为的更灵活,能够产生各种各样的化合物.
- 表观遗传修饰和基因调节显著影响着素生物合成途径的输出.
- 素多基基合成酶表现出复杂的编程选择性,可以调节以产生各种代谢物.
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