一种非排放的 (III) 复合物,专门照亮活细胞的细胞核
Chunyan Li1, Mengxiao Yu, Yun Sun
1Joint Center of Biomedical Imaging (BMI) of Department of Chemistry & Institute of Biomedical Science & Cancer Hospital, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, P. R. China.
Journal of the American Chemical Society
|June 21, 2011
概括
一种新型的 (III) 复合物,LIr1,作为一种基于反应的光探针,可以选择性地照亮活细胞核. 这种药物为核成像提供了一个独特的机制,具有低细胞毒性和高发光度.
科学领域:
- 协调化学 协调化学
- 细胞生物学 细胞生物学
- 生物成像是一种生物成像.
背景情况:
- 循环金属化 (III) 复合物正在用于生物成像应用.
- 现有的核污染通常依赖于与核酸的相互作用,限制了它们的机制.
- 基于反应的探测器的开发提供了增强的特异性和信号放大.
研究的目的:
- 开发一种新型的非发射性 (III) 复合物,用于基于反应的光激活活细胞中的核成像.
- 调查开发的探针的机制,细胞吸收和特异性.
- 评估探测器作为细胞隔间可视化的先进工具的潜力.
主要方法:
- 合成和表征非排放性循环金属化(III) 溶剂复合物[Ir ((ppy) ((2) (((DMSO) ((2))) ((+)) PF ((6))) ((-) (LIr1).
- 活细胞成像实验评估核定位,发光增强和细胞毒性.
- 通过使用各种抑制剂和条件,研究细胞吸收途径.
- 感应合等离子体原子发射光谱 (ICP-AEC) 用于量化核积累.
主要成果:
- LIr1 作为一种基于反应的光激发剂,可选择性地照亮活细胞的细胞核,增强 200 倍的发光强度.
- 该探针在成像度 (10μM,<10分钟化时间) 时表现出低细胞毒性.
- LIr1通过一种能源依赖的途径进入生物细胞,并与核中的含有胺的蛋白质发生反应,产生发光.
结论:
- LIr1是第一个基于反应的光激活剂,用于活细胞中选择性核染色.
- 它基于与核蛋白反应的独特机制,使其与传统的核酸相互作用染料有所区别.
- 这种探测器提供了一种有价值的新工具,用于可视化高特异性和信号增强的活细胞核.
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