氨基林的结构和生物合成,这是来自Amycolatopsis sp.的不寻常的混合配体 siderophore. AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4 AA4
Mohammad R Seyedsayamdost1, Matthew F Traxler, Shao-Liang Zheng
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Journal of the American Chemical Society
|June 25, 2011
概括
罕见的行为菌体,比如Amycolatopsis sp. AA4,产生新的分子. 这项研究确定了氨酸,一种影响菌体发育的 siderophore,揭示了对神秘细菌代谢物的洞察力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 基因组学就是基因组学.
背景情况:
- 动态细菌是多样化的小分子的生产者,具有治疗潜力.
- 基因组数据表明,Actinobacteria可以产生比目前已知的小分子多十倍,突出显示了庞大的"隐秘代谢".
- 从未研究过的细菌中识别新的生物活性化合物对于药物发现至关重要.
研究的目的:
- 为了探索Actinobacteria神秘的二次代谢组.
- 为了研究不同类型的虫虫种类之间的发育相互作用.
- 为了识别和表征由罕见的actinomycetes产生的新型小分子.
主要方法:
- 在20个测序的actinomycetes上进行了对对相互作用测试.
- 用NMR,MS和X射线晶体学分离了氨基,并阐明了它的结构.
- 通过EDTA竞争试验量化铁结合亲和力,并确定了一个生物合成基因集群.
主要成果:
- 艾米科拉托普西斯 (Amycolatopsis sp.) 是一种 AA4是一种罕见的菌体,产生一种名为基林的新型 siderophore.
- 发现氨基林能调节邻近的链状菌株的发育过程.
- 确定了氨酸及其铁复合物的结构,并描述了其铁结合性质.
结论:
- 氨基林代表了一种具有影响细菌发育能力的新型 siderophore.
- 这一发现提供了一种潜在的方法,可以访问罕见的形菌体的神秘代谢组.
- 了解这些相互作用可以导致发现新的治疗药物.
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