转基因小鼠表达人类脊髓灰质炎病毒受体:脊髓灰质炎的新模型
R B Ren1, F Costantini, E J Gorgacz
1Department of Microbiology, College of Physicians and Surgeons Columbia University, New York, New York 10032.
Cell
|October 19, 1990
概括
转基因小鼠表达人类脊髓灰质炎病毒受体 (PVR) 在感染脊髓灰质炎病毒后发展为脊髓灰质炎. 这些PVR小鼠是研究脊髓灰质炎病毒和测试疫苗菌株的宝贵模型.
科学领域:
- 病毒学 病毒学
- 遗传学 是一个遗传学.
- 免疫学 免疫学 免疫学
背景情况:
- 脊髓灰质炎病毒宿主范围由脊髓灰质炎病毒受体 (PVR) 确定.
- 需要小鼠模型来研究脊髓灰质炎病毒的发病和疫苗的疗效.
研究的目的:
- 创建和描述表达人类PVR基因的转基因小鼠.
- 为了评估这些小鼠对脊髓灰质炎病毒感染和疾病的易感性.
主要方法:
- 产生含有人类PVR基因的转基因小鼠.
- 用脊髓灰质炎病毒菌株对PVR转基因和非转基因小鼠进行脑内注射.
- 评估病毒复制,疾病发展和组织热带性.
主要成果:
- 转基因PVR小鼠在大脑和脊髓中支持小儿麻病毒的复制,导致麻性小儿麻症.
- 非转基因小鼠对脊髓灰质炎病毒感染和有抗性.
- 在用减弱的Sabin1型疫苗菌株接种后,PVR转基因小鼠没有发展疾病.
结论:
- 人类PVR基因是小鼠中脊髓灰质炎病毒宿主范围的主要决定因素.
- 转基因PVR小鼠作为宝贵的模型用于脊髓灰质炎病毒研究,包括神经毒性,减弱和疫苗开发.
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