一种合成光遗传转录装置增强了小鼠的血糖平衡
Haifeng Ye1, Marie Daoud-El Baba, Ren-Wang Peng
1Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, Basel, Switzerland.
Science (New York, N.Y.)
|June 25, 2011
概括
科学家们设计了一种新的合成生物学系统,用于哺乳动物细胞的光控制基因表达. 这一突破使得在体内精确远程调节治疗性蛋白质的产生,为治疗提供了新的可能性.
科学领域:
- 合成生物学 合成生物学
- 基因工程是一种基因工程.
- 哺乳动物细胞重编程
背景情况:
- 合成生物学使得遗传装置的设计能够控制细胞功能.
- 控制转基因表达的现有方法在精度和可访问性方面可能受到限制.
研究的目的:
- 设计一种合成信号级联,用于哺乳动物细胞中的光诱导性转基因表达.
- 为了证明在体外和体外治疗性蛋白质生产的远程控制.
主要方法:
- 黑色素信号转导与激活T细胞核因子 (NFAT) 控制电路的功能联系.
- 在细胞培养,生物反应器和动物模型 (小鼠) 中使用可诱导光的转基因细胞.
- 采用光纤和透皮照明来远程控制基因表达.
主要成果:
- 在各种细胞系和小鼠中实现光诱导转基因表达.
- 通过光线远程控制人体糖蛋白分泌的性酸酶血清水平.
- 在II型糖尿病小鼠中展示了光控制的葡萄糖类1表达,以减轻高血糖症.
结论:
- 开发了一种合成光脉冲转录转换器系统,用于精确控制基因表达.
- 这项技术有可能用于治疗应用和蛋白质表达系统.
- 光诱导转基因表达为生物控制和治疗策略提供了一个新的平台.
相关概念视频
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Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
01:16Hormones Regulating Blood Glucose
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...