TGF-β通过自克林PDGF循环的复杂控制来诱导连接组织细胞的双模增殖
E J Battegay1, E W Raines, R A Seifert
1Department of Pathology, University of Washington, Seattle 98195.
Cell
|November 2, 1990
概括
转化生长因子-β (TGF-β) 在细胞生长中表现出双重作用,在低剂量时促进增殖,在较高度时抑制. 这种效应是由连接组织细胞中自身隐性血小板衍生生长因子 (PDGF) 信号传递的.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 已知转化生长因子-β (TGF-β) 调节细胞增殖和分化.
- TGF-β表现出上下文依赖的效果,同时作为增长抑制剂和刺激剂.
- 了解TGF-β对细胞产生作用的精确机制对于细胞生物学研究至关重要.
研究的目的:
- 阐明TGF-β对结缔组织细胞增殖的双相剂量依赖作用.
- 研究血小板衍生生长因子 (PDGF) 在调解TGF-β诱导的增殖中的作用.
- 探索TGF-β对PDGF信号传递的影响的分子机制.
主要方法:
- 细胞培养的光滑肌肉细胞 (SMCs),人体纤维细胞和肌肉细胞.
- 用不同的TGF-β度进行治疗 1.
- 测量DNA合成和细胞增殖.
- 在培养基中检测PDGF-AA和评估PDGF受体α子单元的表达.
主要成果:
- 结缔组织细胞对TGF-β1的双模增殖反应被观察到,最大的增殖在1-2 fg/细胞.
- 低度的TGF-β1诱导了DNA合成的延迟开始,这取决于自身的PDGF-AA分泌.
- 高度的TGF-β1降低了PDGF受体α子单元的表达,并可能直接抑制生长.
结论:
- 在低度下,TGF-β通过自克林PDGF-AA刺激诱导结缔组织细胞的增殖.
- 较高的TGF-β度通过PDGF受体的下调和潜在的直接抑制,导致扩散的减少.
- 这些发现揭示了TGF-β和PDGF信号在调节细胞生长中的复杂相互作用.
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