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相关概念视频

Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Gene Duplication and Divergence02:37

Gene Duplication and Divergence

The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

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相关实验视频

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Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
08:19

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

Published on: December 19, 2011

形态进化是由调节性DNA中的许多微妙效应替代引起的.

Nicolás Frankel1, Deniz F Erezyilmaz, Alistair P McGregor

  • 1Howard Hughes Medical Institute and Department of Ecology and Evolutionary Biology, Princeton University, Princeton, New Jersey 08544, USA.

Nature
|July 2, 2011
PubMed
概括

在Drosophila sechellia的幼虫形态的进化是由于发育基因的变化造成的. 在转录增强剂中的多个单核酸替代改变了基因表达,通过非添加效应引起了显著的形态差异.

科学领域:

  • 进化发育生物学 进化发育生物学
  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学

背景情况:

  • 形态进化是由发育基因的变化驱动的,但因果突变通常是未知的.
  • 在Drosophila sechellia中裸体幼虫皮质的进化与shavenbaby (svb) 基因的转录增强剂的变化有关.

研究的目的:

  • 调查负责SVB增强剂演化的特定单核酸替代.
  • 量化这些核酸替代物对幼虫形态和SVB表达的表型影响.

主要方法:

  • 通过单核酸替代物对特定的svb增强剂的功能分析.
  • 使用新型功能检测方法量化表型后果.
  • 对SVB表达时间和水平的分析.

主要成果:

  • 在SVB增强剂中的多个单核酸替代改变了其功能.
  • 每个替代都有一个小的表型效应,但它们共同导致了显著的形态进化.
  • 替代品对表型表现出非添加效应.

结论:

  • 在转录增强剂中的单个核酸变化可以推动显著的形态进化.

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  • 复杂特征的进化可能是由于小效应突变的积累而产生的,而这些突变具有非添加性相互作用.
  • 提供了对进化变化的遗传基础的高分辨率洞察.