概括
驻留蛋白质通过从cis-Golgi.gi.取回而被保留在内质网膜中. 人类细胞具有类似于酵母的KDEL受体,这表明保存的检索机制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 贩卖蛋白质 贩卖蛋白质 是一个问题.
背景情况:
- 居住在内等质网膜 (ER) 光层中的蛋白质从cis-Golgi或pre-Golgi区块中获取.
- 这种检索是由C端四甲信号介导的:动物细胞中的lys-asp-glu-leu (KDEL) 和酵母中的his-asp-glu-leu (HDEL).
- 酵母ERD2基因被认为编码HDEL分类受体,这是一个26K非糖化成体膜蛋白.
研究的目的:
- 研究人类细胞中KDEL受体的性质.
- 为了确定KDEL检索机制是否在酵母和人类细胞之间保存.
- 挑战哺乳动物细胞中分离的KDEL受体的拟议模型.
主要方法:
- 一种同类于酵母ERD2基因产物的人类蛋白质的表征.
- 分析蛋白质序列,大小和特性.
- 与哺乳动物KDEL受体的现有数据进行比较.
主要成果:
- 人类细胞表达一种蛋白质,其序列,大小和特性与酵母ERD2基因产物相似.
- 这种人类蛋白质被认为是KDEL受体.
- 这些发现表明,KDEL检索机制在物种之间存在保护作用.
结论:
- 人类细胞中的KDEL受体与其酵母对应物具有显著的相似性.
- 这表明ER蛋白质检索的分子机制得到保护.
- 之前建议的分歧哺乳动物KDEL受体模型可能需要重新评估.
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