在2组流感A病毒上高度保存的中和表位
Damian C Ekiert1, Robert H E Friesen, Gira Bhabha
1Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Science (New York, N.Y.)
|July 9, 2011
概括
研究人员发现了一种新的抗体,CR8020,可以中和大多数流感A组2病毒. 这一发现,与现有的抗体一起,可能会导致通用流感疫苗和有效的抗体疗法.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 结构生物学 结构生物学
背景情况:
- 目前的流感疫苗对各种季节性菌株提供有限的保护.
- 之前的突破已经确定了具有广泛中和流感A组1病毒的V(H) 1-69抗体.
研究的目的:
- 隔离和鉴定人类单克隆抗体,对A型流感2组病毒具有广泛的中和活性.
- 为了研究流感病毒血蛋白的抗体识别的结构基础.
主要方法:
- 人类单克隆抗体CR8020的分离和表征.
- 结晶学以确定抗体碎片 (Fab) CR8020与H3血凝素 (HA) 复合体中的结构.
主要成果:
- CR8020对包括H3N2和H7N7.7在内的A型流感2组病毒具有广泛的中和活性.
- 结构分析显示CR8020向HA茎中的一个保存的表位,与V(H) 1-69抗体向不同.
- 已识别的表位在各种流感A亚型中保持一致.
结论:
- CR8020代表了针对A型流感2组病毒的重大进展.
- 结合CR8020和V(H) 1-69抗体可以中和大多数流感A亚型.
- 这种双抗体方法对开发通用流感疫苗和广泛的抗体疗法具有前景.
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