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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
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Published on: March 25, 2009

在Fe3+水溶液中进行超快速杂交选.

Stephan Thürmer1, Robert Seidel, Wolfgang Eberhardt

  • 1Helmholtz-Zentrum Berlin für Materialien und Energie, and BESSY, Albert-Einstein-Strasse 15, D-12489 Berlin, Germany.

Journal of the American Chemical Society
|July 16, 2011
PubMed
概括

这项研究揭示了使用软X射线光电谱学在水性铁 (III) 复合体中的共价混合和电荷转移. 这些发现促进了对过渡金属溶液中电子结构动态的理解.

科学领域:

  • 无机化学 无机化学 有机化学
  • 物理化学 物理化学
  • 频谱学是一种光谱学.

背景情况:

  • 水性过渡金属复合物在各种化学过程中至关重要.
  • 了解它们的电子结构是控制反应性的关键.
  • 软X射线光电子光谱学为电子状态提供了洞察力.

研究的目的:

  • 为了研究FeCl(3) 溶液中的Fe(3+) ((aq) 的电子结合能和电子放松.
  • 为了探测共价混合和电荷转移动态.
  • 为过渡金属溶液建立共振光电子光谱学.

主要方法:

  • 使用真空液体微喷射器进行软X射线光电谱学.
  • 对价值和核心层次光谱的分析.
  • 在铁2p吸收边缘附近的共振增强.
  • 奥格尔衰变光谱学.

主要成果:

  • 直接观察铁3d和水分子轨道之间的共价混合.
  • 在激发的Fe中存在过渡性杂交和电荷转移的证据{3+}{aq) *.
  • 在2p核心孔状态下识别多重相互作用.
  • 在水化中选择性地探测水的电子结构.

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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

Published on: March 25, 2009

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
04:07

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)

Published on: February 23, 2024

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

结论:

  • 软X射线光电子谱学有效地揭示了水溶液中的电子结构和动态.
  • 响应增强是研究金属-连接体相互作用的强大工具.
  • 这项工作为研究各种过渡金属水系统提供了基础.