通过酸化调节孕激素受体介导的转录
L A Denner1, N L Weigel, B L Maxwell
1Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
概括
孕激素受体 (PR) 的酸化对于调节基因转录至关重要. 激活蛋白激酶A (PKA) 或抑制酸酶模仿孕激素.
科学领域:
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
- 基因规则 基因规则
背景情况:
- 孕激素受体 (PR) 是一种调节卵子中的基因转录的蛋白.
- 活体内孕激素治疗刺激PR酸化,这表明它在激素作用中的作用.
研究的目的:
- 研究酸化在调节孕激素受体中介转录中的作用.
- 为了确定蛋白质激酶A (PKA) 和蛋白质酸酶是否参与了这一调节.
主要方法:
- 过渡性转染试验被用来研究PR介导的转录.
- 研究了8-环亚氨酸单酸 (8-Br cAMP) 和酸对转录的作用.
主要成果:
- 8-Br cAMP是一种PKA激活剂,模仿孕激素对转录的影响.
- 抑制PKA阻断了激素的作用.
- 酸,一种酸酶抑制剂,刺激转录类似于孕激素.
结论:
- PR或相关蛋白质的酸化调节了PR介导的转录.
- PKA和蛋白酸酶在调节PR转录活性方面发挥着重要作用.
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