一个集成的半导体装置,使非光学基因组测序成为可能
Jonathan M Rothberg1, Wolfgang Hinz, Todd M Rearick
1Ion Torrent by Life Technologies, Suite 100, 246 Goose Lane, Guilford, Connecticut 06437, USA. Jonathan.Rothberg@Lifetech.com
Nature
|July 22, 2011
概括
这项研究引入了一种新的,非光学DNA测序技术,使用半导体制造进行可扩展,低成本的基因组测序. 离子芯片直接感知DNA合成的副产品,使得更快,更实惠的基因分析.
科学领域:
- 生物技术是生物技术.
- 基因组学就是基因组学.
- 半导体技术 半导体技术
背景情况:
- DNA测序对于生命科学,生物技术和医学至关重要.
- 现有的技术面临着可扩展性和成本方面的挑战.
- 持续需要更高效,更实惠的测序解决方案.
研究的目的:
- 描述一种新的,非光学DNA测序技术.
- 为了利用半导体制造来实现可扩展和低成本的基因组测序.
- 为了证明开发的离子芯片技术的性能和可扩展性.
主要方法:
- 利用互补的金属氧化物半导体 (CMOS) 工艺来制造集成电路.
- 开发了一个带有120万个离子敏感,场效应晶体管基于传感器的离子芯片.
- 采用模板导向的DNA聚合酶合成与天然核酸用于直接的离子传感.
- 进行了细菌和人类基因组的非光学DNA测序.
主要成果:
- 通过使用离子芯片技术成功测序了三种细菌基因组.
- 通过生产具有增加传感器密度 (高达10倍) 的离子芯片,证明了强度和可扩展性.
- 测序了一个完整的人类基因组,展示了系统对大规模应用的能力.
- 实现了DNA合成过程中产生的离子的直接,非光学感应.
结论:
- 开发的基于半导体的DNA测序技术提供了一个可扩展和低成本的解决方案.
- CMOS制造使大规模生产和高密度传感器阵列用于基因组测序成为可能.
- 这种非光学方法具有很大的潜力,可以促进基因组研究,生物技术和医学.
相关概念视频
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