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相关概念视频

Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

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相关实验视频

Updated: May 30, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

一个基于Polycomb的交换机,它是基础定量表观遗传记忆的基础.

Andrew Angel1, Jie Song, Caroline Dean

  • 1Department of Computational and Systems Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

Nature
|July 26, 2011
PubMed
概括
此摘要是机器生成的。

在Vernalization中使用Polycomb镇压复合体2 (PRC2) 来稳定地通过3 lysine 27三甲基化 (H3K27me3) 静止FLC基因. 暴露于寒冷的时间量化控制了这种表观遗传沉默,影响了植物的开花.

更多相关视频

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia
07:10

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

Published on: September 15, 2023

相关实验视频

Last Updated: May 30, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia
07:10

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

Published on: September 15, 2023

科学领域:

  • 表观遗传学和植物生物学
  • 染色体生物学 染色体生物学
  • 基因沉默的分子机制

背景情况:

  • 多镇压复合体2 (PRC2) 通过H3K27me3.3建立了表观遗传沉默.
  • 在阿拉比多普西斯中,化涉及PRC2介导的花抑制器FLC的沉默.
  • 通过PRC2介导的沉默的稳定性和核化仍然是关键的研究问题.

研究的目的:

  • 调查FLC的本土化诱导的表观遗传沉默的定量性质.
  • 为了阐明PRC2核化和稳定性在FLC位点的机制.
  • 了解寒冷暴露时间如何影响稳定的表观遗传记忆.

主要方法:

  • 对表观遗传动力学的数学建模.
  • 染色体免疫沉 (ChIP) 用于量化H3K27me3水平.
  • 一个FLC:GUS记者测试来评估单个细胞中的基因表达模式.

主要成果:

  • 暴露在寒冷中的时间量化地决定了具有稳定的H3K27me3-介导的FLC沉声器的细胞数量.
  • 在冷处理过程中,H3K27me3水平在FLC内的局部核化区域逐渐增加.
  • 建议在FLC位点建立一个双可变的表观遗传切换机制,这取决于寒冷的持续时间,并通过实验验证.

结论:

  • 语言化的定量方面依赖于细胞的亚群实现稳定的H3K27me3沉默FLC.
  • 局部核化H3K27me3标记驱动FLC基因表达中的可视化开关.
  • 这种通过局部组织组织蛋白修饰核化进行表观遗传重编程的机制可能广泛适用.