独立进化的病毒效应因子汇聚到植物免疫系统网络中的枢纽
M Shahid Mukhtar1, Anne-Ruxandra Carvunis, Matija Dreze
1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
概括
植物病原体使用多种多样的毒性蛋白来向高度连接的宿主蛋白,揭示了在不同王国中操纵植物免疫力的保存策略.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 植物通过识别病原体分子来防御病原体.
- 病原体将效应蛋白注入宿主细胞中,以破坏防御.
- 了解效应体-宿主相互作用是植物免疫力的关键.
研究的目的:
- 研究植物病原体作用因子和宿主蛋白之间的相互作用网络.
- 为了确定病原体用来操纵植物免疫力的保存策略.
- 揭示与病原体效应因子相互作用的宿主蛋白的作用.
主要方法:
- 构建一个相互作用网络,涉及两个病原体 (真核生物和真核细菌) 的效应体.
- 整合了关于三类阿拉比多普西斯免疫蛋白和约8000种阿拉比多普西斯蛋白的数据.
- 实验验证植物免疫系统对与效应物相互作用的宿主蛋白的功能.
主要成果:
- 病原体效应体汇聚在高度相互连接的宿主蛋白质 (枢纽) 上.
- 作用因子和植物免疫受体之间的联系在很大程度上是间接的.
- 在17个测试的宿主蛋白中,与效应因子相互作用的15个显示出植物免疫功能.
结论:
- 来自不同王国的病原体独立进化,以针对有限的关键宿主蛋白质枢纽.
- 这种保存的策略促进了病原体生命周期的进展和免疫逃避.
- 针对高度连接的蜂枢纽是植物病原体相互作用中常见的毒性策略.
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