一个ceRNA假设:一个隐藏的RNA语言的罗塞塔石?
Leonardo Salmena1, Laura Poliseno, Yvonne Tay
1Cancer Genetics Program, Beth Israel Deaconess Cancer Center, Departments of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Cell
|August 2, 2011
概括
使者RNA,伪基因和长非编码RNA组成一个竞争的内源RNA (ceRNA) 网络. 这种全转录组的调节网络使用微RNA响应元素 (MREs) 并影响癌症等疾病.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 在RNA生物学,RNA生物学.
背景情况:
- 人类基因组包含了除了蛋白质编码信使RNA (mRNA) 外的多种RNA分子.
- 长非编码RNAs (lncRNAs) 和转录的伪基因代表了转录组的重要部分,其功能在很大程度上是未知的.
- 微RNA (miRNA) 是基因表达的关键调节者,主要通过与miRNA反应元素 (MREs) 结合.
研究的目的:
- 为mRNA,lncRNA和伪基因之间的调节相互作用提出一个统一的假设.
- 引入竞争性内源RNA (ceRNA) 活动的概念,作为一种新的调节机制.
- 突出 ceRNA 网络在人类疾病,特别是癌症中的潜在作用.
主要方法:
- 这项研究主要是以假设为导向的理论框架.
- 它整合了关于RNA-RNA相互作用和miRNA调节的现有知识.
- 作为RNA通信的共同语言,MREs的概念是该假设的核心.
主要成果:
- 使者RNA,转录的伪基因和长非编码RNA可以通过共享的MREs进行相互作用.
- 这种相互作用形成了一个大规模的竞争性内源RNA (ceRNA) 调节网络跨越转录组.
- ceRNA活动显著扩大了人类基因组中编码的功能遗传信息.
结论:
- ceRNA假设为理解转录后调节提供了一个新的框架.
- ceRNA网络代表了一个广泛的调节层,对细胞功能有重大影响.
- 塞RNA网络的失调与包括癌症在内的各种疾病的发病有关,为潜在的治疗点提供了治疗点.
相关概念视频
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...