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Updated: May 30, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

通过二维双差异核Overhauser增强光谱学观察大型蛋白质复合体中的分子间相互作用:应用于44kDa的干扰素受体复合体

Ilona Nudelman1, Sabine R Akabayov, Tali Scherf

  • 1Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel.

Journal of the American Chemical Society
|August 9, 2011
PubMed
概括

这项研究引入了一种新的NMR方法,用于检测大型蛋白质复合体中的分子间相互作用. 该技术增强了信号噪声比,使得蛋白质与蛋白质相互作用的结构研究能够精确地绘制地图.

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High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water08:48

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相关概念视频

Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...