通过两种类型的c-di-GMP рибо开关进行差异化模拟结合
Carly A Shanahan1, Barbara L Gaffney, Roger A Jones
1Department of Chemistry, Yale University, New Haven, Connecticut 06520, United States.
Journal of the American Chemical Society
|August 16, 2011
概括
细菌使用周期性迪加诺辛单酸盐 (c-di-GMP) 来适应. 这项研究揭示了c-di-GMP通过I类和II类 рибо交换机的独特结合机制,其中I类更具选择性.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细菌适应环境变化使用信号分子,如周期性迪加素单酸盐 (c-di-GMP).
- c-di-GMP通过蛋白质受体和基于RNA的核糖开关 (I类和II类) 起作用.
- 之前的研究确定了与c-di-GMP结合的两类 рибо开关的晶体结构.
研究的目的:
- 研究基于RNA的机制来识别和结合第二信使c-di-GMP.
- 确定c-di-GMP的关键结构元素,这些元素对于riboswitch结合至关重要.
- 为了比较I类和II类c-di-GMP核转换器之间的联结差异.
主要方法:
- 使用了一系列周期性迪加诺辛单酸盐 (c-di-GMP) 的类似物.
- 检测了RNA - 连接体复合体内的相互作用,用于两种类型的 рибо开关.
- 分析了c-di-GMP与不同 рибо开关结合的结构要求.
主要成果:
- 对于结合至关重要的c-di-GMP的结构特征在I类和II类 рибо开关之间有所不同.
- 与I类 рибо交换机相比,II类 рибо交换机在连接体结合中的区别明显较低.
- 确定了导致c-di-GMP信号传输中I类 рибо开关流行的差异性结合特异性.
结论:
- RNA发展出不同的策略来结合第二个信使c-di-GMP.
- I类和II类核糖突变器的不同特征为我们提供了关于它们在细菌信号通路中的作用的见解.
- 第I类 рибо开关的较高选择性可能解释了它们在c-di-GMP介导反应中的优先利用.
相关概念视频
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...


