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海胚胎中的细胞相互作用通过光光解研究

C A Ettensohn¹

¹Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213.

Science (New York, N.Y.)

|June 1, 1990

概括

细胞相互作用指导细胞命运. 在海刺胚胎中,初级介质细胞 (PMC) 在胃化晚期影响二级介质细胞 (SMC),在这个关键时期之前,SMC对信号不敏感.

科学领域:

发育生物学是发展生物学.
细胞与细胞的相互作用.
胚胎发生是胚胎发生.

背景情况:

细胞相互作用对于许多生物体的细胞命运规范至关重要.
海胚胎中的初级介质细胞 (PMC) 调节了二级介质细胞 (SMC) 的发育程序.

研究的目的:

确定PMC和SMC之间关键相互作用的精确时间.
了解SMC对PMC信号变得敏感的发展阶段.

主要方法:

采用光光解来选择性地消除不同发育阶段的PMC.
进行PMC微注射到缺少PMC的胚胎中,以评估它们对SMC命运的影响.

主要成果:

PMCs和SMCs之间的关键相互作用是短暂的,并且发生在胃流的晚期.
在这种晚期胃化阶段之前,SMC不会对PMC的抑制信号做出反应.

结论:

细胞对细胞信号传递的发育时间对于细胞命运的决定至关重要.
在海胃化晚期,SMC对PMC介导的发育线索产生敏感性.

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