相关实验视频
Updated: Apr 2, 2026

09:16
Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
9.0K
在核层C上确定细胞周期调节的酸化部位
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|May 18, 1990
概括
成熟促进因子 (MPF) 通过 fosforylating 核层激发核膜在线粒分裂期间的核膜分解. 这种酸化导致了层丝的分解,S6酶II被确定为这个过程中的关键酶.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 分解涉及核膜的分解,这是由成熟促进因子 (MPF) 调节的过程.
- 核层形成支核外的结构网络,是细胞分裂过程中已知的目标.
研究的目的:
- 调查MPF诱导核层分解和核外分解的机制.
- 为了确定核层和涉及的激酶上的特定酸化位.
主要方法:
- 利用青蛋提取物研究MPF添加引起的相间转移到线粒分裂的过渡.
- 使用细菌表达的人类核层C,在体外组装成丝.
- 在MPF的存在和缺席下,使用测序技术分析了层层上的酸化部位.
主要成果:
- 在青蛋提取物中的特定位置,MPF诱导了核层C纤维的酸化增加.
- 观察到层丝片的拆卸与增加的酸化同时发生.
- 识别了化部位的特定氨基酸序列,表明多种酶活动.
- 鉴定出S6激酶II是潜在的关键激酶,它负责层层酸化.
结论:
- 通过MPF介导的酸化是核层分解和核膜在线粒分裂过程中的关键步骤.
- 这些发现突显了特定激酶,包括S6激酶II在细胞分裂过程中调节核结构动态中的作用.
相关概念视频
Positive Regulator Molecules
137.2K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
137.2K
Protein Kinases and Phosphatases
15.6K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.6K
Positive Regulator Molecules
7.1K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
7.1K
M-Cdk Drives Transition Into Mitosis
6.8K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.8K
Disassembly of Intermediate Filaments
2.8K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.8K
MAPK Signaling Cascades
9.2K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
9.2K

