识别一种主要的角质细胞细胞包膜蛋白,loricrinrin
T Mehrel1, D Hohl, J A Rothnagel
1Laboratory of Cellular Carcinogenesis, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Cell
|June 15, 1990
概括
研究人员发现了一种新的蛋白质 - - 洛里克林,它对表皮细胞角化至关重要. 这种蛋白质是细胞外的主要组成部分,对于皮肤屏障形成至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 皮肤病学 皮肤病学
- 蛋白质生物化学 蛋白质生物化学
背景情况:
- 皮表皮细胞角化涉及通过蛋白质交叉链接形成保护性细胞外.
- 了解细胞外的分子成分是皮肤屏障功能的关键.
研究的目的:
- 隔离和表征cDNA克隆编码小鼠表皮细胞的主要分化产物.
- 为了识别和定位参与形成表皮细胞外的蛋白质.
主要方法:
- cDNA克隆和特征鉴定
- 北方斑分析以确定转录本地化和丰富度.
- 针对合成的抗体生产.
- 免疫光和免疫电子显微镜用于蛋白质定位.
主要成果:
- 孤立的cDNA克隆编码具有氨基酸组成的蛋白质,类似于纯化的细胞包裹.
- 基因转录是特定于颗粒层和丰富的,可与质蛋白K1和K10相比较.
- 抗血清将蛋白质定位在层状颗粒体内和层状角质细胞外围的颗粒中.
- 免疫光学和免疫电子显微镜证实了该蛋白质在纯化的细胞包裹的内部表面存在.
结论:
- 鉴定出来的蛋白质名为洛里克林,是表皮细胞外的主要组成部分.
- 洛里克林在角化过程中对皮肤屏障的结构完整性起着重要作用.
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