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相关概念视频

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

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相关实验视频

Updated: May 29, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

通过CTCF的结合元件来控制V(D) J重组的过程.

Chunguang Guo1, Hye Suk Yoon, Andrew Franklin

  • 1Howard Hughes Medical Institute, The Children's Hospital, The Immune Disease Institute, Department of Genetics, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.

Nature
|September 13, 2011
PubMed
概括

研究人员确定了一个新的调节区域,IGCR1,对于免疫球蛋白重链 (IgH) V(D) J重组在小鼠中至关重要. 这一区域通过控制基因段的重新排列,确保了适当的B细胞发育和抗体多样性.

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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

相关实验视频

Last Updated: May 29, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 发育中的B淋巴细胞从V(H),D和J(H) 基因段组装出免疫球蛋白重链 (IgH) 变量区域外子.
  • 2.7兆基鼠标Igh位点需要精确调节针对特定和多样化的抗体谱的V(D) J重组.

研究的目的:

  • 确定和描述小鼠Igh位点中一个新的调节区域,该位点参与V(D) J重组.
  • 阐明该区域在B细胞发育和抗体谱形成中的功能作用.

主要方法:

  • 在小鼠中,V (H) 和D基因集群之间的基因间控制区域1 (IGCR1) 的识别.
  • 使用CTCF循环/绝缘器因子结合元件进行功能分析,并通过远程增强器对Igh循环进行调解.

主要成果:

  • IGCR1抑制了D(H) -近端V(H) 基因段的转录和重新排列,同时促进了远端V(H) 段的重新排列.
  • IGCR1通过防止乳腺细胞的错误结合和调节反来确保有序的V(H) ((D) J(H) 重组.
  • IGCR1对于反调节和近端V(H) 到DJ(H) 重组的等位基排斥至关重要.

结论:

  • IGCR1是免疫球蛋白重链V(D) J重组合的关键调节区域.
  • IGCR1在正常的B细胞发育,抗体库平衡和谱系特异性重组中发挥着关键作用.
  • 这项研究突出了CTCF蛋白在调节Igh位点方面的新功能.