伊塔康酸是一种哺乳动物代谢产物,在巨细胞激活过程中诱导
Cheryl L Strelko1, Wenyun Lu, Fay J Dufort
1Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, USA.
Journal of the American Chemical Society
|September 17, 2011
概括
伊塔康酸 (ITA) 是一种新型代谢物,现在已经在哺乳动物细胞,特别是巨细胞中被发现. 它的产量随着免疫激活而增加,这表明它在免疫反应中起作用.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 代谢学 代谢学 代谢学
背景情况:
- 伊塔康酸 (ITA) 通常不被认为是哺乳动物的代谢产物.
- 巨细胞是参与宿主防御的关键免疫细胞.
研究的目的:
- 研究哺乳动物细胞,特别是巨细胞中ITA的存在和产生.
- 为了阐明这些细胞中ITA的生物合成途径.
主要方法:
- 基于NMR的代谢基因组学
- (13) C-标记的研究.
- 对巨细胞系 (VM-M3,RAW 264.7) 和原发性小鼠巨细胞的分析.
- 用脂聚糖 (LPS) 和干扰素- (IFN-γ) 进行刺激.
主要成果:
- 在巨细胞系和初级巨细胞中检测到ITA.
- 巨细胞激活显著增加了ITA的产生和分泌.
- 原始细胞提取物通过cis-aconitate脱碳化证明了ITA合成,表明一种新的酶活性.
结论:
- 哺乳动物细胞中存在一种以前未知的ITA生物合成途径,与TCA循环代谢有关.
- ITA是一种新型代谢物,可能参与基于巨细胞的免疫反应.
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