与杀手的兼容性解释了缺乏RNAi的真菌的兴起
Ines A Drinnenberg1, Gerald R Fink, David P Bartel
1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
概括
的RNA干扰 (RNAi) 途径.
科学领域:
- 分子生物学分子生物学
- 菌群学 菌群学 菌群学是指菌群学
- 进化生物学 进化生物学
背景情况:
- RNA干扰 (RNAi) 途径是真核生物对病毒和转子体的保护防御机制.
- 在一些真核细胞系中,如Saccharomyces cerevisiae中,RNAi显著缺席.
- 双链RNA (dsRNA) 杀手病毒在真菌中很常见,并提供诸如毒素生产等益处.
研究的目的:
- 为了研究RNAi路径与菌中dsRNA杀手病毒的存在之间的进化关系.
- 了解为什么一些真菌物种缺乏RNAi途径.
主要方法:
- 在Saccharomyces cerevisiae中RNAi途径的重构.
- 具有和没有RNAi和杀手病毒的真菌物种的比较分析.
主要成果:
- 将RNAi重新引入S. cerevisiae导致其有益的dsRNA杀手病毒的丧失.
- 在真菌物种中,RNAi和dsRNA杀手病毒的存在之间观察到强烈的负相关性.
- 具有杀手病毒的真菌物种始终缺乏RNAi,而具有RNAi的相关物种缺乏杀手病毒.
结论:
- RNAi和dsRNA杀手病毒是不相容的,可能是由于病毒干扰RNAi机械.
- 杀手病毒所带来的好处提供了选择性优势,解释了缺少RNAi的真菌系的进化持久性.
- 在某些真菌中缺少RNAi是一种进化适应,以维持有益的病毒感染.
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