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相关概念视频

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

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相关实验视频

Updated: May 29, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

核酸对RNAi的优化:一个高通量平台.

Gabor Butora1, Denise M Kenski, Abby J Cooper

  • 1Department of Process Chemistry, Merck & Co., Rahway, New Jersey 07065, USA. gabor_butora@merck.com

Journal of the American Chemical Society
|September 28, 2011
PubMed
概括
此摘要是机器生成的。

研究人员修改了RNA诱导沉默复合体 (RISC) 中的导向链 (GS) 骨干,以提高siRNA的稳定性和治疗潜力. 这种系统的方法使强效,安全和有效的RNA干扰疗法的合理设计成为可能.

更多相关视频

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

相关实验视频

Last Updated: May 29, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

科学领域:

  • 分子生物学分子生物学
  • 抗RNA干扰疗法 治疗方法
  • 药物发现 药物发现 药物发现

背景情况:

  • RNA诱导沉默复合体 (RISC) 使用导向链 (GS) 进行序列特定的mRNA裂变.
  • 修改GS骨干提供了一个策略来调节RISC活动并改善siRNA属性.

研究的目的:

  • 在RISC的GS内系统地评估改性核化物.
  • 建立一个平台,以合理设计改进的小干扰RNAs (siRNAs).

主要方法:

  • 对于所有四种正规基的色胺的合成与修改.
  • 在21核酸GS的每个位置对修饰核酸的顺序评估.
  • 用伊诺辛作为基线来比较糖修饰核化物.
  • 在特定位置使用2'-O-基修饰验证了平台.

主要成果:

  • 证明GS的位置5,8,15和19可以容纳像2'-O-基这样的大型修改.
  • 建立了一种高通量方法来评估改性核化物.
  • 定义了一个新的活性基线,使用因诺辛替代物.

结论:

  • 开发的高通量方法促进了siRNAs的假设驱动设计.
  • 能够创建强大的,免疫学上无声的,稳定的siRNAs用于治疗.
  • 推动了下一代基于RNA干扰的治疗方法的发展.