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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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一个生物对等的四旋风连接.

Ellen M Sletten1, Carolyn R Bertozzi

  • 1Department of Chemistry and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States.

Journal of the American Chemical Society
|October 4, 2011
PubMed
概括

为了先进的生物研究,开发了一种新的生物对等反应 - - 四旋翼结合. 这种方法使复杂系统中的选择性生物分子修饰成为可能,补充了现有的生物对等工具.

科学领域:

  • 生物对角化学 生物对角化学
  • 化学生物学 化学生物学
  • 生物分子工程 生物分子工程

背景情况:

  • 生物对角化学对于研究生物系统中的生物分子至关重要.
  • 现有的方法在范围和直角性方面存在局限性.
  • 需要新的反应来扩大多重生物分析的工具包.

研究的目的:

  • 介绍和描述一种新的生物对角反应:四旋气连接.
  • 评估反应的效率,动力学和与生物环境的兼容性.
  • 为了证明其与已建立的生物对角化学学的对角性.

主要方法:

  • 四旋旋和Ni bis (乙烯) 试剂之间的四旋结合反应的发展.
  • 动力分析以确定二次速率常数.
  • 在细胞溶解物中选择性标记修饰蛋白的演示.
  • 通过应变促进的亚酸-基环添加和氧基结合进行正交性测试.

主要成果:

  • 四旋翼连接表现出0.25M(-1) s(-1的二次速率常数,相当于快速的生物对角反应.
  • 反应在水性环境中有效地起作用.
  • 通过选择性标记实现了四基兰修饰的牛血清白蛋白,即使存在细胞溶解物.

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  • 四旋旋环结合在一实验中证明了与应变促进的化-基环添加和氧基结合的正交性.

    • 结论:

    • 四旋风结合是一种新的,高效的,生物对角反应.
    • 它扩大了选择性共价改造生物分子的可用工具.
    • 这种反应可以在复杂的生物系统中进行多重分析.